Comprehensive RNA Profiling Across Drosophila Development
A total of 12 RNA samples were collected from different developmental stages and tissues (Supplementary Table S9). The sample sources included adult flies, embryos (eggs), larvae, pupae as well separate tissues including heads, legs, ovipositor, testes, sex organs, and thorax. Total RNA was extracted using the Trizol method and quality-checked as previously described [28 (link)]. The TruSeq stranded library preparation protocol (Illumina) was followed by 100 bp paired-end sequencing with Hiseq 2000/2500 sequencers (Illumina).
Bayega A., Djambazian H., Tsoumani K.T., Gregoriou M.E., Sagri E., Drosopoulou E., Mavragani-Tsipidou P., Giorda K., Tsiamis G., Bourtzis K., Oikonomopoulos S., Dewar K., Church D.M., Papanicolaou A., Mathiopoulos K.D, & Ragoussis J. (2020). De novo assembly of the olive fruit fly (Bactrocera oleae) genome with linked-reads and long-read technologies minimizes gaps and provides exceptional Y chromosome assembly. BMC Genomics, 21, 259.
Other organizations :
McGill University and Génome Québec Innovation Centre, University of Thessaly, Aristotle University of Thessaloniki, Integrated DNA Technologies (United States), University of Patras, International Atomic Energy Agency, Western Sydney University
Tissue sources (heads, legs, ovipositor, testes, sex organs, thorax)
dependent variables
Gene expression levels
control variables
RNA extraction method (Trizol)
RNA quality control
Library preparation protocol (TruSeq stranded)
Sequencing platform (Illumina HiSeq 2000/2500)
controls
Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned
Annotations
Based on most similar protocols
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