Influenza and Pneumococcal Vaccination Study

Five- to six-week-old C57BL/6 and B6.Cg-Lepob/J mice were bled for baseline serum samples, lightly anesthetized with isofluorane, and then vaccinated intramuscularly (n = 5/group) with PBS, 4 hemagglutinating units of β-propriolactone-inactivated, sucrose-purified A/California/04/2009 virus, Pneumovax (Merck), Prevnar (Wyeth), or protein-based YLN vaccine (44 (link)). Prevnar and YLN vaccine were supplemented with alum as an adjuvant. Three weeks following initial vaccination, mice were then boosted with a second dose of vaccine or PBS. Two weeks after the boost, mice were bled for serum collection and then challenged as described above. Serological assessments for influenza virus (42 (link)) and bacterial vaccination (44 (link)) were performed as described previously.

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Karlsson E.A., Meliopoulos V.A., van de Velde N.C., van de Velde L.A., Mann B., Gao G., Rosch J., Tuomanen E., McCullers J., Vogel P, & Schultz-Cherry S. (2017). A Perfect Storm: Increased Colonization and Failure of Vaccination Leads to Severe Secondary Bacterial Infection in Influenza Virus-Infected Obese Mice. mBio, 8(5), e00889-17.

Publication 2017

Pneumovax

Adjuvant Alum Bacterial vaccination California virus Influenza virus Mice Pneumovax Prevnar Protein Second vaccine Serum Sucrose Vaccination Vaccine

Corresponding Organization :

Other organizations : St. Jude Children's Research Hospital, University of Tennessee Health Science Center

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Variable analysis

independent variables

Vaccine type (PBS, β-propriolactone-inactivated, sucrose-purified A/California/04/2009 virus, Pneumovax, Prevnar, protein-based YLN vaccine)

dependent variables

Serum antibody levels against influenza virus
Serum antibody levels against bacterial vaccination

control variables

Mouse strain (C57BL/6 and B6.Cg-Lepob/J)
Mouse age (5- to 6-week-old)
Anesthesia (isofluorane)
Route of vaccination (intramuscular)
Timing of vaccination (initial vaccination and booster dose)

positive controls

PBS (for vaccine groups)

negative controls

Not explicitly mentioned

Annotations

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