On day 0, C57BL/6J mice between 8–10 weeks of age were ovariectomised to deplete endogenous steroid production. Mice received daily injections of β-oestradiol (E2) in sesame seed oil (100 ng/100 µl, days 7–9). A progesterone (P4)-secreting pellet was placed sub-cutaneously on day 13; mice also received daily injections of sub-cutaneous injections of E2 (5 ng/100 µl, days 13–15). On day 15, decidualisation of one uterine horn was induced by stimulation of the horn using sesame seed oil (20 µl) inserted into the uterine lumen via the cervix using a non-surgical embryo transfer device (NSET) from Datesand Ltd. (Manchester, UK). The contra-lateral horn acted as a control. P4 withdrawal was induced 90 hours after decidualisation by removing the P4-pellet. Mice were culled by asphyxiation and cervical dislocation at time of P4 withdrawal or 4, 8, 12, 24 and 48 hours thereafter (
Menstruation Mouse Model with Non-Surgical Induction
On day 0, C57BL/6J mice between 8–10 weeks of age were ovariectomised to deplete endogenous steroid production. Mice received daily injections of β-oestradiol (E2) in sesame seed oil (100 ng/100 µl, days 7–9). A progesterone (P4)-secreting pellet was placed sub-cutaneously on day 13; mice also received daily injections of sub-cutaneous injections of E2 (5 ng/100 µl, days 13–15). On day 15, decidualisation of one uterine horn was induced by stimulation of the horn using sesame seed oil (20 µl) inserted into the uterine lumen via the cervix using a non-surgical embryo transfer device (NSET) from Datesand Ltd. (Manchester, UK). The contra-lateral horn acted as a control. P4 withdrawal was induced 90 hours after decidualisation by removing the P4-pellet. Mice were culled by asphyxiation and cervical dislocation at time of P4 withdrawal or 4, 8, 12, 24 and 48 hours thereafter (
Corresponding Organization :
Other organizations : University of Edinburgh
Protocol cited in 11 other protocols
Variable analysis
- Ovariectomy to deplete endogenous steroid production
- Daily injections of β-oestradiol (E2) in sesame seed oil (100 ng/100 µl, days 7–9)
- Progesterone (P4)-secreting pellet placed sub-cutaneously on day 13
- Daily injections of sub-cutaneous injections of E2 (5 ng/100 µl, days 13–15)
- Decidualisation of one uterine horn induced by stimulation of the horn using sesame seed oil (20 µl) inserted into the uterine lumen via the cervix
- P4 withdrawal induced 90 hours after decidualisation by removing the P4-pellet
- Uterine tissue collection during a period of active shedding and repair
- Cellular proliferation detected by bromodeoxyuridine (BrdU) incorporation
- Contra-lateral uterine horn as a control
- Contra-lateral uterine horn as a negative control
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