EGFR Signaling Pathway Activation Assay

Human recombinant EGF was from BD Bioscience. EGF-Rh was from Invitrogen. Monoclonal antibody to EGFR phosphotyrosine 1068 (pY1068), polyclonal antibody to α-actinin, polyclonal antibody to ERK1/2 and monoclonal antibody to phosphorylated ERK1/2 were from Cell Signaling Technology (Beverly, MA). EGFR monoclonal antibody 04–105 were from Transduction Laboratories. PD158780 was from Calbiochem. MG-B-Tau and MG-Bis-SA were synthesized by the Bruchez lab (Perkins et al., 2018b (link); Yan et al., 2015 (link)). All chemicals were from ThermoFisher unless stated otherwise.

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Larsen M.B., Perez Verdaguer M., Schmidt B.F., Bruchez M.P., Watkins S.C, & Sorkin A. (2019). Generation of endogenous pH-sensitive EGF receptor and its application in high-throughput screening for proteins involved in clathrin-mediated endocytosis. eLife, 8, e46135.

Publication 2019

Human recombinant EGF EGF-Rh

Actinin Antibody Egfr Erk1 Human Monoclonal antibody Pd158780 Phosphotyrosine

Corresponding Organization :

Other organizations : University of Pittsburgh, Imaging Center, Carnegie Mellon University, Sharp Edge Labs (United States)

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Variable analysis

independent variables

Human recombinant EGF from BD Bioscience
EGF-Rh from Invitrogen
PD158780 from Calbiochem
MG-B-Tau and MG-Bis-SA synthesized by the Bruchez lab

dependent variables

EGFR phosphotyrosine 1068 (pY1068) measured by monoclonal antibody
α-actinin measured by polyclonal antibody
ERK1/2 and phosphorylated ERK1/2 measured by polyclonal and monoclonal antibodies

control variables

All chemicals from ThermoFisher unless stated otherwise

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

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