Western Blot Protein Analysis

Western blot was performed as previously described^{30 (link)}. GAPDH was used as a loading control. Uncropped blots can be seen in Supplemental Fig. 4. Antibodies and dilutions used are as follows:
GAPDH (Cell Signaling, 2118, 1:2000)
Phospho-STAT3 (Cell Signaling, 9131, 1:1000)
STAT3 (Cell Signaling, 30835, 1:1000)
HE4 (Origene, TA307787, 1:2000)
HIF1A (Novus, NB-100–134SS, 1:200)

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James N.E., Emerson J.B., Borgstadt A.D., Beffa L., Oliver M.T., Hovanesian V., Urh A., Singh R.K., Rowswell-Turner R., DiSilvestro P.A., Ou J., Moore R.G, & Ribeiro J.R. (2020). The biomarker HE4 (WFDC2) promotes a pro-angiogenic and immunosuppressive tumor microenvironment via regulation of STAT3 target genes. Scientific Reports, 10, 8558.

Publication 2020

GAPDH Phospho-STAT3 STAT3 HIF1A

Antibodies Dilutions Gapdh Hif1a Novus Seen Stat3 Western blot

Corresponding Organization :

Other organizations : Women & Infants Hospital of Rhode Island, Providence College, Rhode Island Hospital, Northwell Health, University of Rochester Medical Center, Brown University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Phosphorylation of STAT3
Expression of STAT3
Expression of HE4
Expression of HIF1A

control variables

GAPDH expression (used as a loading control)

controls

Positive control: None mentioned
Negative control: None mentioned

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