Bone Density and Microarchitecture Analysis in Mice

Bone mineral density (BMD) was measured in live mice by dual-energy x-ray absorptiometry with a PIXImus Mouse Densitometer (GE Lunar Corp., Madison, WI) using the manufacturer’s software as described previously18 (link). Growth curves were obtained by sequential BMD measurement of the same animals from 8 weeks to 24 weeks of age. Micro-CT analysis of cortical and trabecular architecture was performed in femurs and fourth lumbar vertebrae (L4), as previously described19 (link). Biomechanical properties of femurs and L4 vertebrae were measured by 3-point bending and compression testing respectively, as previously described20 (link). L1–L3 lumbar vertebrae were fixed, embedded undecalcified in methylmethacrylate, and histomorphometric examination was done on longitudinal sections with a digitizer tablet (OsteoMetrics, Inc., Decatur, GA) interfaced to a Zeiss Axioscope (Carl Zeiss, Thornwood, NY) with a drawing tube attachment, as previously described21 (link). Terminology recommended by the Histomorphometry Nomenclature Committee of the American Society for Bone and Mineral Research was used in this study22 (link).

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Piemontese M., Onal M., Xiong J., Han L., Thostenson J.D., Almeida M, & O’Brien C.A. (2016). Low bone mass and changes in the osteocyte network in mice lacking autophagy in the osteoblast lineage. Scientific Reports, 6, 24262.

Publication 2016

digitizer tablet Zeiss Axioscope

Animals Bone Bone mineral density Cortical Dual energy x ray absorptiometry Femurs Lumbar vertebrae Methylmethacrylate Micro ct Mineral Mouse Tablet Trabecular Vertebrae

Corresponding Organization :

Other organizations : University of Arkansas for Medical Sciences, Central Arkansas Veterans Healthcare System

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Variable analysis

independent variables

Age of mice (8 weeks to 24 weeks)

dependent variables

Bone mineral density (BMD) measured by dual-energy x-ray absorptiometry
Cortical and trabecular architecture of femurs and fourth lumbar vertebrae (L4) measured by micro-CT analysis
Biomechanical properties of femurs and L4 vertebrae measured by 3-point bending and compression testing
Histomorphometric examination of L1-L3 lumbar vertebrae

control variables

Mice used in the study

controls

No positive or negative controls were explicitly mentioned in the protocol.

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