All tissues were fixed in formalin-acetic acid-alcohol (FAA) for 24 h at 4°C, subsequently embedded in paraffin, and sectioned to a thickness of 4 μm. HE staining was performed following standard histological procedures [38 (link)]. IF staining was performed as previously described [39 (link)]. The primary antibodies used for the IF were as follows: mouse anti-GRP78 (1:100, sc-376768, Santa Cruz Biotechnology, Santa Cruz, USA), rabbit anti-BCL2 (1:50, ab32124, Abcam, Cambridge, UK), mouse anti-Beclin1 (1:50, NBP1-00085) rabbit anti-p62 (1:200, NBP1-49955, Novus, Littleton, USA) and FOXL2 (1:50, 19672-1-AP, Proteintech, Wuhan, China). Sections were viewed under an Eclipse 80i microscope (Nikon, Tokyo, Japan). The fluorescence images of the slides were visualized using an IX70 fluorescence microscope (OLYMPUS, Tokyo, Japan). The tissue stained without adding primary antibody was considered to be a negative control.
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