Cryptococcal isolates studied in this study are listed in Appendix Tables 1 and 2 . The isolates were obtained by the participating laboratories of the IberoAmerican Cryptococcal Study Group and maintained on Sabouraud dextrose agar slants at 4°C and as water cultures at room temperature. Isolates were identified as C. neoformans by using standard methods (20 ). The variety was determined by the color reaction test on L -canavanine-glycine-bromothymol blue medium (21 (link)), and the serotype was determined, in selected isolates, by the use of the Crypto Check Kit (Iatron Laboratories Inc., Tokyo, Japan).
The isolates were sent for molecular typing to the Molecular Mycology Laboratory at the University of Sydney at Westmead Hospital, Sydney, Australia, either as water cultures or on Sabouraud dextrose agar slants. For long-term storage, the isolates were maintained as glycerol stocks at –70°C.
The isolates were sent for molecular typing to the Molecular Mycology Laboratory at the University of Sydney at Westmead Hospital, Sydney, Australia, either as water cultures or on Sabouraud dextrose agar slants. For long-term storage, the isolates were maintained as glycerol stocks at –70°C.
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