Molecular Signaling Regulation by Chi3l1

Recombinant mouse proteins (Chi3l1, rIL13Rα2, RANKL, and M-CSF) were provided by R&D systems (USA). The Gibco BRL (USA) provided minimal essential medium alpha (α-MEM), fetal bovine serum (FBS), 0.25% EDTA-trypsin, and penicillin-streptomycin. RiboFECTTM transfection reagent, Chi3l1-siRNA, and IL13Rα2-siRNA came from RiboBio company (China). Primary antibodies targeting p-Akt (Ser473), Akt, p-ERK, ERK, p-JNK, JNK, p-P38, P38, p-P65, P65, and GAPDH were obtained from Cell Signaling Technology (CST, USA). The Abcam (USA) provided the primary antibody recognizing Chi3l1, which was used for immunofluorescence (IF) and western blot (WB). The primary antibody that recognized Chi3l1 used for co-immunoprecipitation (IP) and IL13Rα2 for IF and WB came from R&D systems (USA). Santa Cruz (USA) offered the primary antibody that recognized IL13Rα2 for IP.

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Xu W., Chao R., Xie X., Mao Y., Chen X., Chen X, & Zhang S. (2024). IL13Rα2 as a crucial receptor for Chi3l1 in osteoclast differentiation and bone resorption through the MAPK/AKT pathway. Cell Communication and Signaling : CCS, 22, 81.

Publication 2024

Chi3l1 RANKL M-CSF α-MEM GAPDH

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University, Shanghai Stomatological Hospital, Shanghai Ninth People's Hospital, National Clinical Research

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Variable analysis

independent variables

Recombinant mouse proteins (Chi3l1, rIL13Rα2, RANKL, and M-CSF)

dependent variables

Not explicitly mentioned

control variables

Minimal essential medium alpha (α-MEM)
Fetal bovine serum (FBS)
0.25% EDTA-trypsin
Penicillin-streptomycin
RiboFECTTM transfection reagent
Chi3l1-siRNA
IL13Rα2-siRNA
Primary antibodies targeting p-Akt (Ser473), Akt, p-ERK, ERK, p-JNK, JNK, p-P38, P38, p-P65, P65, and GAPDH
Primary antibody recognizing Chi3l1 for immunofluorescence (IF) and western blot (WB)
Primary antibody recognizing Chi3l1 for co-immunoprecipitation (IP)
Primary antibody recognizing IL13Rα2 for IF and WB
Primary antibody recognizing IL13Rα2 for IP

controls

Not explicitly mentioned

Annotations

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