Human PC cell lines (AsPC-1, Capan-2 and BxPC-3) were cultured as previously described5 (link). The three cell lines display different genetic profiles5 (link). Human fibroblast cell line HFF-1 was purchased from American Type Culture Collection (ATCC; Manassas, VA, USA) and it was cultured in DMEM high glucose (4.5 g/L), supplemented with 15% FBS.
8-Hydroxy-5-nitroquinoline (nitroxoline) was purchased from Sigma (St. Louis, MO, USA). All compounds were dissolved in DMSO (stock solutions of 40 mM) and then in culture media for the final working concentrations. The final concentration of DMSO in the experiments was at most 0.27% and showed no cell toxicity.
8-Hydroxy-5-nitroquinoline (nitroxoline) was purchased from Sigma (St. Louis, MO, USA). All compounds were dissolved in DMSO (stock solutions of 40 mM) and then in culture media for the final working concentrations. The final concentration of DMSO in the experiments was at most 0.27% and showed no cell toxicity.
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