Protocol detail

Diatom Retinal Quantification by LC-MS/MS

Find Similar Protocols

Retinal concentrations in the diatom isolates and field samples were determined by liquid-chromatography tandem mass spectrometry (LC–MS/MS) according to refs. (11 (link), 15 (link)). The LC–MS/MS apparatus consisted of a Thermo Accela™ LC system (refrigerated autosampler and LC pump) coupled to a Thermo TSQ™ Quantum Access™ heated electrospray ionization triple quadrupole mass spectrometer. Further detailed methods for retinal quantification are provided in supplemental information. Retinal-to-C ratios of the diatom isolates were estimated by dividing cellular retinal concentrations by cellular C concentrations, as determined using a Costech 4010 CHNOS elemental combustion system.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Andrew S.M., Moreno C.M., Plumb K., Hassanzadeh B., Gomez-Consarnau L., Smith S.N., Schofield O., Yoshizawa S., Fujiwara T., Sunda W.G., Hopkinson B.M., Septer A.N, & Marchetti A. (2023). Widespread use of proton-pumping rhodopsin in Antarctic phytoplankton. Proceedings of the National Academy of Sciences of the United States of America, 120(39), e2307638120.

Publication 2023

Accela™ LC system TSQ™ Quantum Access™ 4010 CHNOS

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Retinal concentrations in the diatom isolates and field samples
Retinal-to-C ratios of the diatom isolates

control variables

None explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!