Quantitative Gene Expression Analysis in Maize

For Real-time quantitative reverse transcription PCR (Real-time PCR) analyses, the first-strand cDNA was synthesized using TransScript® First-Strand cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China). Real-time PCR was performed using SYBR Green Mix (TIANGEN, Beijing, China) in a LightCycler96 instrument (Roche, Switzerland), with the maize GAPDH gene as the internal reference. The ABA signaling pathway genes ZmSnRK2.4, ZmPP2C-A4, ZmPP2C-A7, ZmRD17, ZmRAB18, and ZmLEA were selected for expression validation using specific primers [25 (link)].

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Li Y., Su Z., Lin Y., Xu Z., Bao H., Wang F., Liu J., Hu S., Wang Z., Yu X, & Gao J. (2024). Utilizing transcriptomics and metabolomics to unravel key genes and metabolites of maize seedlings in response to drought stress. BMC Plant Biology, 24, 34.

Publication 2024

TransScript® First-Strand cDNA Synthesis SuperMix SYBR Green Mix LightCycler96

Corresponding Organization : Inner Mongolia Agricultural University

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Variable analysis

independent variables

Primers for ABA signaling pathway genes (ZmSnRK2.4, ZmPP2C-A4, ZmPP2C-A7, ZmRD17, ZmRAB18, and ZmLEA)

dependent variables

Expression levels of ABA signaling pathway genes (ZmSnRK2.4, ZmPP2C-A4, ZmPP2C-A7, ZmRD17, ZmRAB18, and ZmLEA)

control variables

GAPDH gene as the internal reference

controls

Positive control: Not specified
Negative control: Not specified

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