To assess catalase activity, a heated dichromate in acetic acid solution was added in presence of H2O2. This mixture underwent a transformation into perchromic acid and finally to chromic acetate. The formation of chromic acetate was measured at a wavelength of 570 nm [56 (link)].
Antioxidant Enzyme Activity Assay
To assess catalase activity, a heated dichromate in acetic acid solution was added in presence of H2O2. This mixture underwent a transformation into perchromic acid and finally to chromic acetate. The formation of chromic acetate was measured at a wavelength of 570 nm [56 (link)].
Corresponding Organization :
Other organizations : Vidyasagar University
Variable analysis
- Ovarian tissues were homogenized in Tris-HCl buffer (pH 7.4)
- SOD (superoxide dismutase) activity was measured at a wavelength of 340 nm
- Formation of chromic acetate was measured at a wavelength of 570 nm
- Tris-HCl buffer (pH 7.4)
- Thio barbituric acid (TBA)
- 7.5 mmol/L nicotinamide adenine dinucleotide phosphate-reduced
- Nicotinamide adenine dinucleotide phosphate-reduced-MnCl2
- Heated dichromate in acetic acid solution
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