Ovarian tissues were homogenized in Tris-HCl buffer (pH 7.4), and the resulting supernatant was mixed with thio barbituric acid (TBA), 7.5 mmol/L nicotinamide adenine dinucleotide phosphate-reduced, nicotinamide adenine dinucleotide phosphate-reduced-MnCl2. The SOD (superoxide dismutase) activity was then measured at a wavelength of 340 nm [55 (link)].
To assess catalase activity, a heated dichromate in acetic acid solution was added in presence of H2O2. This mixture underwent a transformation into perchromic acid and finally to chromic acetate. The formation of chromic acetate was measured at a wavelength of 570 nm [56 (link)].
To assess catalase activity, a heated dichromate in acetic acid solution was added in presence of H2O2. This mixture underwent a transformation into perchromic acid and finally to chromic acetate. The formation of chromic acetate was measured at a wavelength of 570 nm [56 (link)].
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