Genomic Analysis of C. culicis and P. polymyxa

Genomic DNA of C. culicis and P. polymyxa was extracted using the SDS method. Then, the DNA was measured using agarose gel electrophoresis and assessed using a Qubit®2.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). Purified genomic DNA was used to construct a sequencing library with the NEB Next® Ultra^TM DNA Library Prep Kit (NEB, Beverly, MA, USA). The genomes of C. culicis and P. polymyxa were sequenced on the Nanopore PromethION platform and Illumina NovaSeq PE150 (Beijing Novogene Bioinformatics Technology Co., Ltd, Beijing, China). After trimming low-quality reads using fastq, clean reads were assembled using SPAdes 3.13.1^{59 (link),81 (link)}. Bioinformatics analysis focused on KEGG Orthology^{82 (link)}. Genome overviews were created by Circos to reveal the annotations^{83 (link)}.