Blood Oxygen Level-Dependent (BOLD)-MRI was performed to evaluate myocardial oxygenation18 (link)-22 (link). Briefly, under 1-2% isoflurane anesthesia, pigs were positioned in the MRI scanner (Signa Twin Speed EXCITE 3T system, GE Healthcare, Waukeshau, WI) and BOLD images (4-5 axial-obeselique) acquired along the cardiac short axis during suspended respiration. Gated Spoiled Gradient Echo (SPGR) sequence with TR/TE/number of echoes/Matrix size/FOV/Slice thickness/Flip cardiac angle = 20.5 ms/2 ms/8/128x128/35/0.5 cm/30°. Imaging was repeated during intravenous injection of adenosine (400 μg/kg/min) through an ear vein catheter. BOLD data was processed in MATLAB 7.10 (The MathWorks Inc., Natick, MA). The BOLD index, R2*, was estimated in each voxel by fitting the MR signal intensity vs. echo time to a single exponential function and calculating the MR intensity decay rate. Myocardial R2* values were estimated from regions of interest (ROI) defined based on T2*-weighted MR images and calculating R2* weighted-average of all ROIs.
Protocol detail
Myocardial Oxygenation Assessment via BOLD-MRI
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Adenosine
Anesthesia
Axis
Blood oxygen level
Cardiac
Catheter
Echo
Isoflurane
Ms ms
Myocardial
Pigs
Respiration
Twin
Vein
Corresponding Organization : Mayo Clinic
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Variable analysis
independent variables
- Adenosine administration (400 μg/kg/min)
- Myocardial R2* (MR intensity decay rate)
- Isoflurane anesthesia (1-2%)
- Gated Spoiled Gradient Echo (SPGR) sequence parameters (TR/TE/number of echoes/Matrix size/FOV/Slice thickness/Flip cardiac angle)
- BOLD imaging during suspended respiration
- Positive control: Adenosine injection to induce myocardial vasodilation and change in BOLD signal
- Negative control: Not explicitly mentioned
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