Generation of TCR-transduced CD4+ T Cells
Corresponding Organization : Chongqing Medical University
Other organizations : First Affiliated Hospital of Chongqing Medical University
Variable analysis
- Stimulation of CD4+ T cells with 1 μg/ml OKT3 antibody and 1 μg/ml anti-CD28 antibody
- Transduction of CD4+ T cells and CD4+ Jurkat cells with lentivirus
- Outcome of subsequent assays performed on the TCR-transduced-CD4+ T and TCR-transduced-CD4+ Jurkat cells
- Culture of CD4+ T cells in complete medium containing 100 IU/ml IL-2 for 48 h
- Incubation of CD4+ Jurkat cells and stimulated CD4+ T cells with 500 μl lentivirus and 500 μl RPMI 1640 for 6 h
- Replacement of original medium with 2-ml complete medium and incubation for 48 h
- Not explicitly mentioned
- Not explicitly mentioned
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