Biotinylated RNA Pull-Down Assay

The biotinylated RNA pull-down experiment was implemented according to previous studies [57 (link)]. Biotinylated circMYOF probes were synthesized and labelled by a TranscriptAid T7 Kit (Thermo Fisher Scientific, USA) in accordance with the manufacturer’s instructions. Then, probe-coated beads were produced using Pierce^TM streptavidin magnetic beads (Thermo Fisher Scientific, USA). Cells were collected and then hatched with streptavidin magnetic beads at 4 °C for 4 h. Afterwards, the binding RNA was isolated with RNAiso Plus. RNA bands were analysed by agarose gel electrophoresis.