Surgical Anesthesia and Cannula Implantation in Rat NTS

A combination of ketamine (75 mg/kg; Ketaminol Vet, Intervet International BV, AN Boxmeer, the Netherlands) and xylazine (10 mg/kg; Rompun Vet, Bayer Animal Health GmbH, Leverkusen Germany) were IP administered to achieve surgical anaesthesia. Guide cannulas (26‐gauge; Plastics One, Roanoke, VA, USA) were implanted above the nucleus of the solitary tract (NTS) using coordinates adapted from Richard et al.³⁵ The NTS cannula was implanted at the following coordinates: ±0.75 mm from the midline/on occipital suture/–4.9 mm, with the injector aimed 6.9 mm ventral to skull.

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Asker M., Krieger J., Liles A., Tinsley I.C., Borner T., Maric I., Doebley S., Furst C.D., Börchers S., Longo F., Bhat Y.R., De Jonghe B.C., Hayes M.R., Doyle R.P, & Skibicka K.P. (2023). Peripherally restricted oxytocin is sufficient to reduce food intake and motivation, while CNS entry is required for locomotor and taste avoidance effects. Diabetes, Obesity & Metabolism, 25(3), 856-877.

Publication 2023

Rompun Vet

Anaesthesia Animal Cannula Ketamine Nucleus of the solitary tract Rompun Skull Surgical Suture Xylazine

Corresponding Organization : Pennsylvania State University

Other organizations : Syracuse University, University of Pennsylvania, SUNY Upstate Medical University

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