HPLC analysis GSSG formation was performed using
a Hitachi Primaide instrument equipped with a C18 column (Xbridge
Peptide BEH C18 column from Waters, 4.6 mm × 150 mm, pore size
300 Å, particle size 3.5 μm), using 0.1% aqueous TFA (solvent
A) and 90% CH3CN/0.1% TFA in water (solvent B) with a linear gradient
from 5% to 10% solvent B in 7 min.
a Hitachi Primaide instrument equipped with a C18 column (Xbridge
Peptide BEH C18 column from Waters, 4.6 mm × 150 mm, pore size
300 Å, particle size 3.5 μm), using 0.1% aqueous TFA (solvent
A) and 90% CH3CN/0.1% TFA in water (solvent B) with a linear gradient
from 5% to 10% solvent B in 7 min.
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