Collecting Honey Bee Worker Samples

A single healthy and well-established honey bee colony, headed by a Carniolan Apis mellifera carnica queen, was used as a source for all worker samples of this study. Using sister bees originating from a single colony helps minimize variability in hive conditions and the genetic make-up of the workers. A total of eight capped worker brood frames ready to hatch were removed from this hive and placed in an incubator at 35 °C with 50–60% relative humidity. The following day, several thousand one-day-old sister bees were collected into a sterile plastic box for further use.

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Alburaki M., Madella S, & Cook S.C. (2023). Non-optimal ambient temperatures aggravate insecticide toxicity and affect honey bees Apis mellifera L. gene regulation. Scientific Reports, 13, 3931.

Publication 2023

Apis Bees Frames Genetic Hive Honey Humidity Sterile Worker

Corresponding Organization : Agricultural Research Service

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Colony headed by a Carniolan Apis mellifera carnica queen
Hive conditions and genetic makeup of the workers minimized by using sister bees originating from a single colony
Temperature at 35 °C with 50–60% relative humidity in the incubator

controls

Positive control: Not specified
Negative control: Not specified

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