Co-culture of RMF and vHMEC for RNA Isolation

RMF were plated in 0.4μm pore Transwell dishes (Costar) and co-cultured with vHMEC expressing either control luciferase short hairpin (sh-Luciferase), activin A short hairpin (sh-activin A), vector control, TRF2 or hTERT (19 (link), 20 (link)). RNA was isolated from RMF 48 hours after initiation of co-culture.

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DeFilippis R.A., Fordyce C., Patten K., Chang H., Zhao J., Fontenay G.V., Kerlikowske K., Parvin B, & Tlsty T.D. (2014). Stress Signaling from Human Mammary Epithelial Cells Contributes to Phenotypes of Mammographic Density. Cancer research, 74(18), 5032-5044.

Publication 2014

Transwell dishes

Activin a Co culture Dishes Luciferase

Corresponding Organization : University of California, San Francisco

Other organizations : Lawrence Berkeley National Laboratory, University of Nevada, Reno

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Variable analysis

independent variables

VHMEC expressing either control luciferase short hairpin (sh-Luciferase), activin A short hairpin (sh-activin A), vector control, TRF2 or hTERT

dependent variables

RNA isolated from RMF 48 hours after initiation of co-culture

control variables

RMF plated in 0.4μm pore Transwell dishes (Costar) and co-cultured with vHMEC

controls

Positive control: vHMEC expressing control luciferase short hairpin (sh-Luciferase)
Negative control: vHMEC expressing vector control

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