Scotopic ERG in C57BL/6J Mice

Scotopic electroretinography (ERG) was conducted in 12-week old WT C57BL/6 J mice before and after STF31 treatment using Espion Visual Electrophysiology system (Diagnosys LLC, Cambridge, UK) as described previously [23 (link)]. Briefly, mice were anesthetized and pupils dilated as above. The mouse was placed on the heat-pad (38 °C). ERG was recorded using mouse corneal ERG electrodes in response to single white light flash, delivered by a standard Ganzfeld Stimulator (Diagnosys LLC). ERG signals were bandpass filtered between 0.3–500 Hz (without notch filtering), amplified 500-fold and digitized at 2 kHz using the CMGS-1 electrophysiology system (Diagnosys LLC).

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Wang L., Pavlou S., Du X., Bhuckory M., Xu H, & Chen M. (2019). Glucose transporter 1 critically controls microglial activation through facilitating glycolysis. Molecular Neurodegeneration, 14, 2.

Publication 2019

Espion Visual Electrophysiology system

C57bl mice Corneal Electroretinography Light Mouse Pupils

Corresponding Organization :

Other organizations : Queen's University Belfast

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Variable analysis

independent variables

STF31 treatment

dependent variables

Scotopic electroretinography (ERG) responses

control variables

Mouse strain (WT C57BL/6 J mice)
Mouse age (12-week old)
Anesthesia
Pupil dilation
Mouse body temperature (38 °C)
Electrodes (mouse corneal ERG electrodes)
Light stimulus (single white light flash, delivered by Ganzfeld Stimulator)
ERG signal processing (bandpass filtered between 0.3–500 Hz, without notch filtering, amplified 500-fold, digitized at 2 kHz)

controls

Positive control: Baseline ERG responses before STF31 treatment
Negative control: Not explicitly mentioned

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