Sequencing of Lactococcus lactis N8-8 Genome

Isolation of gDNA was carried out using SDS method. Total DNA obtained was subjected to quality control by agarose gel electrophoresis and quantified by Qubit (Thermo Fisher Scientific, Waltham, MA, USA). The genome of L. lactis N8-8 was sequenced with MPS (massively parallel sequencing) Illumina technology (Illumina, San Diego, CA, USA). The DNA library was constructed: a paired-end library with an insert size of 350 bp. The 350-bp library was sequenced using an Illumina PE150 strategy. Library construction and sequencing were performed at the Beijing Novogene Bioinformatics Technology Co., Ltd. Quality control of paired-end reads was performed using in-house program. Then, data processing, reads mapping and SV (structural variation) analysis were conducted as we described previously [12 (link)].