Quantifying Ascorbate Levels via HPLC

Ascorbate concentrations in cell lysates, tissues, and plasma were measured using reverse-phase high-performance liquid chromatography (HPLC; Waters 600 HPLC; Waters, MA, USA) with an ESA Coulochem II detector (Thermo Fisher Scientific), as described before.19 (link),22 (link),23 (link) A fresh standard curve of 0–40 μM ascorbate was prepared for each HPLC run.

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Campbell E.J., Vissers M.C, & Dachs G.U. (2016). Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo−/− mice. Hypoxia, 4, 41-52.

Publication 2016

Waters 600 HPLC

Cell Hplc Plasma Reverse phase liquid chromatography Tissues

Corresponding Organization :

Other organizations : University of Otago

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Variable analysis

independent variables

Ascorbate concentrations

dependent variables

Ascorbate concentrations in cell lysates, tissues, and plasma

control variables

A fresh standard curve of 0–40 μM ascorbate was prepared for each HPLC run

controls

Positive control: Not specified
Negative control: Not specified

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