Primary HUVECs were obtained from Cambrex Bio Science (Charles City, IA, USA) and were maintained as described previously41 (link), 42 (link). Briefly, cells were cultured in EBM-2 basal media supplemented with growth supplements (Cambrex Bio Science, Charles City, IA, USA) at 37 °C under a 5% CO2 atmosphere until confluent. All experiments were performed with HUVECs at passage 3–5.
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