Pupil Area Tracking Methodology

Pupil area was tracked as previously described in Burgess et al. (2017) (link). Briefly, a camera (DMK 21BU04.H or DMK 23U618, The Imaging Source) with a zoom lens (ThorLabs MVL7000) was focused on one of the eyes of the animal. The eye was illuminated by an infrared LED (SLS-0208A, Mightex). Videos of the eye were acquired at ≥30 Hz. In each video frame, excluding frames with blinks, an ellipse was fit to the pupil image, and pupil area was estimated based on this fit.

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Okun M., Steinmetz N.A., Lak A., Dervinis M, & Harris K.D. (2019). Distinct Structure of Cortical Population Activity on Fast and Infraslow Timescales. Cerebral Cortex (New York, NY), 29(5), 2196-2210.

Publication 2019

DMK 21BU04.H DMK 23U618 MVL7000 SLS-0208A

Animal Blinks Frames Lens Pupil

Corresponding Organization : University College London

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Pupil area

control variables

Camera (DMK 21BU04.H or DMK 23U618, The Imaging Source)
Zoom lens (ThorLabs MVL7000)
Infrared LED (SLS-0208A, Mightex) for eye illumination
Video frame capture rate of ≥30 Hz
Exclusion of frames with blinks

controls

None explicitly mentioned

Annotations

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