B. lactis BL-99 was deposited in the China
Common Microbial Culture Collection and Management Center (CGMCC 15650) on April
26, 2018 [29 (link)] and
identified by 16S rRNA gene amplified using the universal primers 27F
(5’-AGA GTT TGA TCC TGG CTC AG-3’ ) and 1492R
(5’-GGT TAC CTT GTT ACG ACT T T-3’ ) [45 (link)]. The standard
B. lactis BL-99 culture was proliferated
with De Man Rogosa Sharpe (MRS) medium (Solarbio, Beijing) supplemented with
0.05% (w/v) L-cysteine (MRSC) for 12–48 hrs at 37°C aerobically [26 (link)] and the anaerobic
environment was obtained with Anaero Gen sachets (Oxoid Ltd., West
Heidelberg/Vic., Australia). Colony-forming unit (CFU) of B.
lactis BL-99 was 1.5*1011 CFU / g and preserved
at -80°C.
Common Microbial Culture Collection and Management Center (CGMCC 15650) on April
26, 2018 [29 (link)] and
identified by 16S rRNA gene amplified using the universal primers 27F
(
(
B. lactis BL-99 culture was proliferated
with De Man Rogosa Sharpe (MRS) medium (Solarbio, Beijing) supplemented with
0.05% (w/v) L-cysteine (MRSC) for 12–48 hrs at 37°C aerobically [26 (link)] and the anaerobic
environment was obtained with Anaero Gen sachets (Oxoid Ltd., West
Heidelberg/Vic., Australia). Colony-forming unit (CFU) of B.
lactis BL-99 was 1.5*1011 CFU / g and preserved
at -80°C.
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