Immunofluorescence Staining of Cell Markers

Cells were fixed in 4% paraformaldehyde, and immunofluorescence staining was performed as described previously.^{11 (link)} Antibodies against DAXX, E-cadherin, vimentin, CD44 and HDAC-1 were purchased from Cell Signaling (1/100). Antibodies against CD24 were purchased from Abcam (1/100). Goat anti-rabbit IgG—Alexa Fluor^® 568 conjugate and goat anti-mouse IgG—Alexa Fluor^®488 conjugate were purchased from Thermo Fisher Scientific (Waltham, MA). DAPI was added at the final step to reveal nuclei.

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Wu C., Ding H., Wang S., Li Y., Liu S.B., Wang X., Zheng J., Xue T., Amin H.M., Song Y.H, & Zhou J. (2020). DAXX inhibits cancer stemness and epithelial–mesenchymal transition in gastric cancer. British Journal of Cancer, 122(10), 1477-1485.

Publication 2020

E-cadherin vimentin HDAC-1 Goat anti-rabbit IgG—Alexa Fluor® 568 conjugate goat anti-mouse IgG—Alexa Fluor®488 conjugate

Alexa 568 Alexa fluor 488 Anti igg Antibodies Cd44 Cells Dapi Daxx E cadherin Goat Immunofluorescence Mouse Nuclei Paraformaldehyde Rabbit Vimentin

Corresponding Organization : First Affiliated Hospital of Soochow University

Other organizations : Suzhou Vocational Health College, The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Antibodies against DAXX, E-cadherin, vimentin, CD44 and HDAC-1
Antibodies against CD24

dependent variables

Immunofluorescence staining

control variables

Cells were fixed in 4% paraformaldehyde
DAPI was added at the final step to reveal nuclei

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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