The tyrosinase inhibition assay was measured as described by Neely et al. (2009) [42 (link)]. Each 1 mL assay contained a final concentration of 100 mM sodium phosphate (pH 6.5) and 2 mM L-DOPA. Finally, 0.2 mg/mL of mushroom tyrosinase solution (Sigma-Aldrich) was added to the mixture. Control, with an equal amount of extraction solvent replacing the extract, was routinely carried out. Reaction processes were traced by using a microplate reader (BioTek ELX800; BioTek Instruments Inc., Winooski, VT, USA) at a wavelength of 475 nm. The tyrosinase inhibitory effect was expressed as a % of inhibition relative to the corresponding control for each extract. Standard compound concentrations applied in a study were 100 µM and the concentration of tested extracts was 50 µg/mL. The experiments were repeated three times and the average results with standard deviation values were given in Figure 3 B.
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