Dental Pulp Stem Cell Neural Differentiation

The neural differentiation from dental pulp stem cells was obtained as reported in our previous study^{5 (link)}. Briefly, cells were cultured for 6 days in vitro (DIV) in the basic medium (DMEM), subsequently the medium was added with the following neural induction cocktail: 0.5 mM Isobutyl Methyl Xanthine (IBMX), 20 ng/ml human Epidermal Growth Factor (hEGF) 1 mM dibutyrylcAMP (dbcAMP), 10 ng/ml Nerve Growth Factor (NGF) and 10 ng/ml Brain-Derived Neurotrophic Factor (BDNF), 40 ng/ml basic Fibroblastic Growth Factor (bFGF), (all reagents were purchased from Invitrogen, Milan, Italy). Obtained neuronal differentiation, cells were grown in culture for 15 days in DMEM/FBS added with 10 µM retinoic acid²¹.

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Bonaventura G., Iemmolo R., La Cognata V., Zimbone M., La Via F., Fragalà M.E., Barcellona M.L., Pellitteri R, & Cavallaro S. (2019). Biocompatibility between Silicon or Silicon Carbide surface and Neural Stem Cells. Scientific Reports, 9, 11540.

Publication 2019

Brain-Derived Neurotrophic Factor (BDNF basic Fibroblastic Growth Factor (bFGF),

Basic fibroblastic growth factor Brain derived neurotrophic factor Cells Dental pulp Epidermal growth factor Human Methyl xanthine Nerve growth factor Neural Neural stem cells Neuronal

Corresponding Organization :

Other organizations : Institute for Biomedical Research and Innovation, National Research Council, Institute for Microelectronics and Microsystems, University of Catania

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Variable analysis

independent variables

Neural induction cocktail: 0.5 mM Isobutyl Methyl Xanthine (IBMX), 20 ng/ml human Epidermal Growth Factor (hEGF) 1 mM dibutyrylcAMP (dbcAMP), 10 ng/ml Nerve Growth Factor (NGF) and 10 ng/ml Brain-Derived Neurotrophic Factor (BDNF), 40 ng/ml basic Fibroblastic Growth Factor (bFGF)

dependent variables

Neuronal differentiation

control variables

Cells were cultured for 6 days in vitro (DIV) in the basic medium (DMEM) before addition of the neural induction cocktail
Obtained neuronal differentiation, cells were grown in culture for 15 days in DMEM/FBS added with 10 µM retinoic acid

controls

No positive or negative controls were explicitly mentioned in the protocol.

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