ChIP assay was performed using the chip-IT kit (Active Motif), following the manufacturer’s instructions and as described elsewhere (81 (link)). E2F3 binding on the PGC-1α promoter was calculated by subtracting the intensity value of the band detected upon immunoprecipitation with the anti-E2F3 antibody from the intensity value detected from the input band, and the difference is presented as percentage arbitrary units.
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