Characterization of C. glabrata Mutants

The C. glabrata luminescent strain SSY21 was pre-grown for UTI infection as described [26 (link)]. Deletion and insertion mutants were pre-grown in yeast peptone dextrose (YPD) (2% Bacto Peptone, 1% yeast extract and 2% glucose) at 30°C under continuous shaking. Spot assays were performed on nitrogen starvation (NS) medium (0,17% YNB without amino acids and without ammonium sulphate, 2% glucose, 2% Bacto agar) supplemented with either 0.5% Urea (PlusOne, GE healthcare), 5 mM L-Citrulline (Sigma) or 5 mM L-Tryptophan (Sigma) for DUR1,2 and GAP1 mutants and on YNB (0.67% YNB without amino acids, 2% Bacto agar) supplemented with either 2% potassium acetate or 2% ethanol for MLS1 and VMA22 mutants. All strains are summarized in Table S1.