The hepatic tissue from zebrafish was excised surgically and processed, as mentioned in Section 2.6 . The hepatic morphological changes and ROS production were examined by H&E and DHE staining, respectively, as mentioned in Section 2.6 .
The pro-inflammatory IL-6 level in the hepatic tissue was determined by immunohistochemical (IHC) staining as previously described method [36 (link)]. In brief, the tissue section was flooded with 200× diluted IHC primary antibody (ab9324, Abcam, London, UK), followed by overnight incubation at 4 °C. The EnVision+ System-HRP polymer kit containing secondary antibody (1:1000, Code K4001, Dako, Denmark) developed the IHC stained area that was visualized under an ocular microscope (Nikon, Tokyo, Japan).
The pro-inflammatory IL-6 level in the hepatic tissue was determined by immunohistochemical (IHC) staining as previously described method [36 (link)]. In brief, the tissue section was flooded with 200× diluted IHC primary antibody (ab9324, Abcam, London, UK), followed by overnight incubation at 4 °C. The EnVision+ System-HRP polymer kit containing secondary antibody (1:1000, Code K4001, Dako, Denmark) developed the IHC stained area that was visualized under an ocular microscope (Nikon, Tokyo, Japan).
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