Mouse Spinal Cord Injury Model

The mouse SCI model was established as previously described [51 (link)]. Briefly, male C57BL/6J mice of 12 weeks of age were anesthetized with 330 mg/kg of 2-2-2 Tribromoethanol (Sigma) intraperitoneally and undertook a laminectomy at the T12 vertebrae. Then a contusion SCI (hemitransection) was induced on the exposed dorsal surface of dura mater through using a SCI micro-scissor devise (Infinite Horizon impactor at 60 kDyn, Precision Systems and Instrumentation, USA). Sham group includes the complete procedures without hemitransection of the spine. During recovery, mice were maintained in an isothermic cage. For animal welfare, all animal experimental procedures were approved by the Ethics Committee of the third hospital, Hebei Medical University.