Total RNA Isolation from Minced Tendon

RNA was isolated as modified from previous studies (Gumucio et al., 2014 (link); Grinstein et al., 2018 (link); Disser et al., 2019 (link)). Tendons were finely minced and then pulse homogenized in Qiazol (Qiagen, Germantown, MD, USA) containing 1μg of glycogen (Qiagen) using a TissueRuptor (Qiagen). RNA was isolated with an miRNA Micro Kit (Qiagen) supplemented with DNase I (Qiagen), and quality was assessed using a BioAnalyzer RNA Pico kit (Agilent Technologies, Santa Clara, CA, USA).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Disser N.P., Ghahramani G.C., Swanson J.B., Wada S., Chao M.L., Rodeo S.A., Oliver D.J, & Mendias C.L. (2020). Widespread diversity in the transcriptomes of functionally divergent limb tendons.. The Journal of physiology, 598(8), 1537-1550.

Publication 2020

Qiazol glycogen TissueRuptor miRNA Micro Kit DNase I BioAnalyzer RNA Pico kit

Dnase i Glycogen Mirna Pulse Tendons

Corresponding Organization : Cornell University

Top 5 similar protocols

Variable analysis

independent variables

Isolation of RNA from tendons using a modified protocol

dependent variables

RNA quality and yield

control variables

Glycogen added during RNA isolation (1μg)
Use of Qiazol for tissue homogenization
Use of TissueRuptor for homogenization
Use of miRNA Micro Kit with DNase I treatment for RNA isolation
Use of BioAnalyzer RNA Pico kit for RNA quality assessment

controls

No explicit positive or negative controls are mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!