CDK8/CDK19 single knockout and double knockout derivatives of human embryonic kidney (HEK) 293 cells (ATCC CRL-1573) were generated as previously described [18 (link)]. All cells were cultured in DMEM (high-glucose) media supplemented with 10% fetal bovine serum (FBS) and Penicillin-Streptomycin-Glutamine (1×) at 37 °C with 5% CO2 and routinely confirmed to be free of mycoplasma. Microscopic examination was carried out by Olympus CKX41 Inverted Phase Contrast Microscope and the cell images were captured and processed with CellSens standard software.
Human recombinant TNFα (Z00404-50) was obtained from GenScript (Piscataway, NJ, USA). Senexin B and 15w were provided by Senex Biotechnology (Columbia, SC, USA). Didehydro-cortistatin A (dCA) was a gift from Phil S. Baran (Scripps Research Institute, La Jolla, CA, USA). Cmpd3 (CCT251921) and Cmpd4 (MSC2530818) were a gift from Merck KGaA, Darmstadt, Germany. Recombinant human epidermal growth factor (EGF) and transforming growth factor beta (TGFβ) proteins were obtained from R&D Systems, Minneapolis, MN, USA. Other reagents were obtained from MilliporeSigma, St. Louis, MO, USA.
Human recombinant TNFα (Z00404-50) was obtained from GenScript (Piscataway, NJ, USA). Senexin B and 15w were provided by Senex Biotechnology (Columbia, SC, USA). Didehydro-cortistatin A (dCA) was a gift from Phil S. Baran (Scripps Research Institute, La Jolla, CA, USA). Cmpd3 (CCT251921) and Cmpd4 (MSC2530818) were a gift from Merck KGaA, Darmstadt, Germany. Recombinant human epidermal growth factor (EGF) and transforming growth factor beta (TGFβ) proteins were obtained from R&D Systems, Minneapolis, MN, USA. Other reagents were obtained from MilliporeSigma, St. Louis, MO, USA.
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