IHC analysis and H-scoring of the FFPE tissue sections was performed as described earlier [57 (link)]. Images were captured using an Olympus BX61 microscope using image pro plus software (Olympus, Center Valley, PA, USA) at 10 × and 20 × optical magnifications. The human samples contained a collection of grade II, III and IV tumor sections (n = 38) with adjacent normal portions wherever possible.
For ICC, cells were seeded on coverslips and processed as described earlier [52 (link)]. Epifluorescence images were captured using an Olympus BX61 microscope andimage pro plus software (Olympus) and confocal images with an Andor spinning disc confocal microscope (Andor Technology, Belfast, UK) and Andor iXon 8797 EMCCD camera using andor iq 2 software, both at 60 × optical magnification.
For ICC, cells were seeded on coverslips and processed as described earlier [52 (link)]. Epifluorescence images were captured using an Olympus BX61 microscope and
