The surface markers of cultured C-MSC are characterized by flow cytometry analyses with a BD LSRII flow cytometer and BD FACSDiva™ software as previously described[27 (link)]. Briefly, C-MSCs were blocked with 5% rat serum and stained with a panel of conjugated antibodies, including anti-CD105-APC (BioLegend), anti-CD140-PE (eBioscience) and anti-CD117-FITC (BD Biosciences Pharmingen).
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Characterization of C-MSC Surface Markers
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Other organizations : Zhongda Hospital Southeast University, Augusta University
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Variable analysis
independent variables
- Blocking with 5% rat serum
- Staining with a panel of conjugated antibodies, including anti-CD105-APC, anti-CD140-PE, and anti-CD117-FITC
- Surface markers of cultured C-MSC characterized by flow cytometry analyses
- C-MSCs
- BD LSRII flow cytometer
- BD FACSDiva™ software
- No positive or negative controls were explicitly mentioned.
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