Immunoblotting to Quantify Protein Levels

To monitor the changes in the protein levels of FGF9, ki-67, MMP-2, and MMP-9, we performed immunoblotting following the methods described before [55 (link)] with antibodies against CD206, CD16, FGF9, ki-67, MMP-2, and MMP-9 (ab64693, ab46679, ab71395, ab15580, and ab38898, Abcam, Cambridge, UK) followed by another incubation with the appropriate HRP-conjugated secondary antibodies. GAPDH (ab16891, Abcam) was used as an endogenous control. Signals were visualized using enhanced chemiluminescent (ECL) substrates (WBKlS0100, Millipore, MA, USA) and were normalized to the GAPDH signal.

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Wu S., Xu R., Zhu X., He H., Zhang J., Zeng Q., Wang Y, & Zhao X. (2020). The long noncoding RNA LINC01140/miR-140-5p/FGF9 axis modulates bladder cancer cell aggressiveness and macrophage M2 polarization. Aging (Albany NY), 12(24), 25845-25864.

Publication 2020

ab64693 ab46679 ab71395 ab15580 ab38898 ab16891 WBKlS0100

Antibodies Fgf9 Gapdh Mmp 2 Mmp 9 Protein changes

Corresponding Organization :

Other organizations : Second Xiangya Hospital of Central South University, Central South University

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Variable analysis

independent variables

Antibodies against CD206
Antibodies against CD16
Antibodies against FGF9
Antibodies against ki-67
Antibodies against MMP-2
Antibodies against MMP-9

dependent variables

Protein levels of FGF9
Protein levels of ki-67
Protein levels of MMP-2
Protein levels of MMP-9

control variables

GAPDH as an endogenous control

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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