Lentiviral shRNA vectors targeting human FoxO1 and FoxO3 were described in our previous publication (18 (link)). Rictor retroviral vector and Lentiviral shRNA vector targeting human Rictor were described in the previous publication (38 (link)). NVP-BEZ235 was purchased from LC Laboratories. BKM120 was ordered from ChemieTek. MK2206 was ordered from Selleck. 4-OHT was purchased from Sigma. The following antibodies were used in this study: Vinculin (Sigma), FoxO1 (C29H4), FoxO3 (75D8), phospho-FOXO1(Thr24)/FOXO3(Thr32), S6, Ser240/244 phospho-S6, Rictor, AKT, Ser473 phospho-AKT, Thr308 phospho-AKT, GSK3, phospho-GSK3, cleaved caspase-3, Ki67, ERBB3 (all from Cell Signaling Technology).
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Investigating FoxO, Rictor, and AKT Pathways
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Corresponding Organization : The University of Texas MD Anderson Cancer Center
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Variable analysis
independent variables
- Lentiviral shRNA vectors targeting human FoxO1
- Lentiviral shRNA vectors targeting human FoxO3
- Rictor retroviral vector
- Lentiviral shRNA vector targeting human Rictor
- NVP-BEZ235
- BKM120
- MK2206
- 4-OHT
- Vinculin
- FoxO1
- FoxO3
- Phospho-FOXO1(Thr24)/FOXO3(Thr32)
- Ser240/244 phospho-S6
- Rictor
- Ser473 phospho-AKT
- Thr308 phospho-AKT
- Phospho-GSK3
- Cleaved caspase-3
- ERBB3
- Vinculin
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