Western blotting procedures were previously described (26 (link)). Primary antibodies included: anti-NEDD9 mAb (2G9) (19 (link)), anti-NEDD9 (p55, custom made using NEDD9 1–394aa as an antigen), anti-β-actin mAb, or anti-GAPDH (Sigma), anti-AURKA (BD Biosciences), anti-AURKA (AurA-N, custom made using 1–126aa N-terminal fragment of AURKA), anti-phospho-T288Aurora A (Cell Signaling), anti-Histone H3 and anti-phospho-Ser10 Histone H3 and anti-Ubiquitin (Millipore, BD Biosciences). Blots were developed by the HyGLO HRP Detection Reagent (Denville Scientific, Inc.). Bands were digitized and quantified using a digital electrophoresis documentation and image analysis system (G-box, Syngene Corp.).
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Western Blot Analysis of Cell Signaling Proteins
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Other organizations : West Virginia University
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Variable analysis
independent variables
- Anti-NEDD9 mAb (2G9)
- Anti-NEDD9 (p55, custom made using NEDD9 1–394aa as an antigen)
- Anti-β-actin mAb
- Anti-GAPDH (Sigma)
- Anti-AURKA (BD Biosciences)
- Anti-AURKA (AurA-N, custom made using 1–126aa N-terminal fragment of AURKA)
- Anti-phospho-T288Aurora A (Cell Signaling)
- Anti-Histone H3
- Anti-phospho-Ser10 Histone H3
- Anti-Ubiquitin (Millipore, BD Biosciences)
- Bands were digitized and quantified using a digital electrophoresis documentation and image analysis system (G-box, Syngene Corp.)
- Western blotting procedures were previously described (26 (link))
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