Western blotting was performed as described previously (Pu and Zhao, 2005 (link)). Primary antibodies used were: anti-ALPI (NOVUS); anti-Ror2 (Abcam); anti-pERK1/2, anti-ERK anti-LKB1, anti-pLKB1 and anti-pERM (Cell Signaling); anti-GFP (Abcam); and anti-ezrin and anti-GAPDH (Santa Cruz Biotechnology). For inhibitor experiments, cells were pre-incubated with 50 µM U0126 (Cell Signaling), 30 µM ouabain or 30 µM digoxin (Sigma-Aldrich) for the time indicated.
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