Radioimmunoprecipitation assay lysis buffer (Sangon) was applied for total protein extraction, followed by Western blot detection referring to the issued description.16 (link)
The primary Abs targeting B-cell lymphoma-2 (Bcl-2; ab32124, 1:1000), Bcl-2-associated X (Bax; ab182733, 1:1000), RORA (ab256799, 1:1000), GAPDH (ab181603, 1:1000) were provided by Abcam (Cambridge, UK). Goat Anti-rabbit IgG H&L (HRP) secondary Ab (Abcam, ab205718, 1:5000) was incubated at 25°C for 1 h, followed by analysis of protein bands through ECL luminescence reagent (Sangon) and Image J software (NIH, Bethesda, MD, USA).
The primary Abs targeting B-cell lymphoma-2 (Bcl-2; ab32124, 1:1000), Bcl-2-associated X (Bax; ab182733, 1:1000), RORA (ab256799, 1:1000), GAPDH (ab181603, 1:1000) were provided by Abcam (Cambridge, UK). Goat Anti-rabbit IgG H&L (HRP) secondary Ab (Abcam, ab205718, 1:5000) was incubated at 25°C for 1 h, followed by analysis of protein bands through ECL luminescence reagent (Sangon) and Image J software (NIH, Bethesda, MD, USA).
