Spectrophotometric Method for Thiol-Disulfide Assessment

An spectrophotometric method which Erel and Neselioglu described was used to measure thiol/disulfide Homeostasis tests [14 (link)]. Disulfide bonds are reduced and combined with sodium borohydride, and then, thiol groups are formed. The reductant sodium borohydride which remained unused was consumed and removed with formaldehyde to prevent reducing DTNB (5.5’-dithiobis-(2-nitrobenzoic) acid), and after the reaction with DTNB, all of the thiol groups, including native and reduced thiol groups, were specified. If the natural thiols are subtracted from the total thiols, half of the difference represents the dynamic sulfur content. After native and total thiols were determined, disulfide/native thiol percent ratios (SS/SH), disulfide/total thiol percent ratios (SS/SH+SS), disulfide amounts, and native thiol/total thiol percent ratios (SH/SH+SS) were determined [15 (link)].

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Eroglu N., Sahin G., Yesil S., Fettah A., Yildiz Y.T, & Erel O. (2023). Thiol disulfide homeostasis in ionizing radiation and chemotherapeutic drug exposure. Northern Clinics of Istanbul, 10(1), 53-58.

Publication 2023

Disulfide Dtnb Formaldehyde Homeostasis Nitrobenzoic acid Reductant Sodium borohydride Spectrophotometric Sulfur Thiol

Corresponding Organization :

Other organizations : Dr Sami Ulus Çocuk Sağlığı ve Hastalıkları Eğitim ve Araştırma Hastanesi, Ankara Yıldırım Beyazıt University

Top 5 similar protocols

Variable analysis

independent variables

Reduction of disulfide bonds with sodium borohydride
Removal of unused sodium borohydride with formaldehyde

dependent variables

Measurement of thiol/disulfide homeostasis
Determination of native thiols
Determination of total thiols
Calculation of disulfide/native thiol percent ratios (SS/SH)
Calculation of disulfide/total thiol percent ratios (SS/SH+SS)
Calculation of disulfide amounts
Calculation of native thiol/total thiol percent ratios (SH/SH+SS)

control variables

Reaction with DTNB (5.5'-dithiobis-(2-nitrobenzoic) acid) to measure all thiol groups, including native and reduced thiol groups

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!