Protocols for Exploring Drug Resistance in WM Cells

WM cell lines and derived bortezomib-resistant (BR) suclones,^{24 (link), 25 (link)} as well as ibrutinib-resistant (IR) subclones,^{26 , 27 (link)} were used in experiments, as reported previously.^{23 (link), 25 (link)} Primary WM patient cells (CD19+/CD138+ sorted) were obtained from the Predolin Biobank (Mayo Clinic, Rochester MN, USA) after approval by the Mayo Clinic Institutional Review Board. Heparinized peripheral blood from healthy human donors was obtained and peripheral blood mononuclear cells (PBMCs) were extracted, as described previously.^{28 (link)} All cells were cultured according to conditions previously described by us.^{23 (link)} VLX1570 and b-AP15 were provided as gifts from Vivolux AB (Uppsala, Sweden). RPMI, penicillin, streptomycin, tetramethylrhodamine, methyl ester (TMRM) and fetal bovine serum were purchased from Life Technologies (Carlsbad, CA, USA). Ibrutinib and bortezomib were purchased from Sellekhem (Houston, TX, USA). Annexin-V/Propidium Iodide Apoptosis Staining Kit was purchased from BD Biosciences (San Jose, CA, USA).

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Paulus A., Akhtar S., Caulfield T.R., Samuel K., Yousaf H., Bashir Y., Paulus S.M., Tran D., Hudec R., Cogen D., Jiang J., Edenfield B., Novak A., Ansell S.M., Witzig T., Martin P., Coleman M., Roy V., Ailawadhi S., Chitta K., Linder S, & Chanan-Khan A. (2016). Coinhibition of the deubiquitinating enzymes, USP14 and UCHL5, with VLX1570 is lethal to ibrutinib- or bortezomib-resistant Waldenstrom macroglobulinemia tumor cells. Blood Cancer Journal, 6(11), e492-.

Publication 2016

RPMI penicillin streptomycin methyl ester (TMRM) fetal bovine serum Annexin-V/Propidium Iodide Apoptosis Staining Kit

Annexin v Apoptosis Bortezomib Cd138 Cell lines Cells Ester Fetal bovine serum Gifts Human Ibrutinib Institutional review board Patient Penicillin Peripheral blood donors Peripheral blood mononuclear cells Propidium iodide Streptomycin Tetramethylrhodamine Vlx1570

Corresponding Organization :

Other organizations : Mayo Clinic in Florida, WinnMed, Mayo Clinic in Arizona, Cornell University, Karolinska Institutet

Top 5 similar protocols

Variable analysis

independent variables

WM cell lines and derived bortezomib-resistant (BR) subclones
Ibrutinib-resistant (IR) subclones

dependent variables

Measured outcomes

control variables

Primary WM patient cells (CD19+/CD138+ sorted)
Peripheral blood mononuclear cells (PBMCs) from healthy human donors

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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