Immunofluorescence Staining of Telomeric Proteins

IF experiments were carried out as previously described (Nandakumar et al., 2012 (link)), using the following antibodies: mouse monoclonal anti-TRF2 (IMG-124A; 1:500; Imgenex), rabbit polyclonal anti-coilin (sc-32860; 1:100; Santa Cruz, Dallas, TX), rat monoclonal anti-mCherry (M11217; 1:1000; Life Technologies), and rabbit polyclonal anti-RAP1 (NB-100-292; 1:500; Novus Biologicals, Littleton, CO). Secondary antibodies (Life Technologies, Abcam) were pre-absorbed to prevent cross-reactivity between rat and mouse antibodies.

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Schmidt J.C., Dalby A.B, & Cech T.R. (2014). Identification of human TERT elements necessary for telomerase recruitment to telomeres. eLife, 3, e03563.

Publication 2014

IMG-124A sc-32860

Antibodies Biologicals Cross reactivity Mouse Novus Rabbit

Corresponding Organization :

Other organizations : Howard Hughes Medical Institute, University of Colorado Boulder

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Variable analysis

independent variables

Antibodies used: mouse monoclonal anti-TRF2, rabbit polyclonal anti-coilin, rat monoclonal anti-mCherry, and rabbit polyclonal anti-RAP1

dependent variables

Not explicitly mentioned

control variables

Experiments were carried out as previously described (Nandakumar et al., 2012)

controls

Secondary antibodies were pre-absorbed to prevent cross-reactivity between rat and mouse antibodies (positive control)

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