Determination of serum SOD activity was performed through the concept given by the scientists In which quercetin undergoes oxidation by O2.33 ,34 (link) Standard solutions were prepared by adding 0.8 mM tetramethylethylenediamine (TMEDA) and 0.08 mM EDTA together in a clean tube of buffer solution (pH = 9) containing 16 mM potassium sulfate. Then, 100 μL of serum and 20 μL of quercetin were added together, and after 20 minutes, the optical density was measured by a spectrophotometer at 406 nm.
Trace minerals (zinc and copper) were measured colorimetrically according to the manufacturer’s instructions (Randox, UK). The measurements of Vitamin C, catalase activity, GPx activity, and TAC were achieved after following the steps of the manufacturer (Elabscience, USA).
Trace minerals (zinc and copper) were measured colorimetrically according to the manufacturer’s instructions (Randox, UK). The measurements of Vitamin C, catalase activity, GPx activity, and TAC were achieved after following the steps of the manufacturer (Elabscience, USA).
