A total of 1 × 105 conidia were inoculated per well in 24-well plates containing sterile coverslips and cultured for 8 h in liquid RPMI 1640 media (Wisent) containing doxycycline at the indicated concentrations. Coverslips were washed with PBS, stained with 50 μg/ml of fluorescein-tagged soybean agglutinin lectin (SBA-FITC), fixed in 4% paraformaldehyde, mounted, and imaged as described above, with an excitation of 495 nm and an emission of 519 nm. A series of images in the z-plane were obtained to encompass whole hyphae and converted into maximum intensity projections using ZEN black edition software (Zeiss). The MFI of the stained hyphae was quantified by measuring the mean pixel density using ImageJ software (60 (link)).
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