Detecting MmuPV1 Viral Transcripts using RNAscope

MmuPV1 viral transcripts were detected using RNAscope 2.5 HD Assay-Brown (Advanced Cell Diagnostics, Newark, CA) according to the manufacturer’s instructions (61 (link)) with probes specific for MmuPV1 E1^E4 (catalog no. 473281). Tissue sections were treated following protease treatment and prior to probe hybridization with 20 units of DNase I (Thermo Fisher Scientific; catalog no. EN0521) for 30 min at 40°C. Slides were counterstained with hematoxylin before mounting and coverslipping.

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Spurgeon M.E., Uberoi A., McGregor S.M., Wei T., Ward-Shaw E, & Lambert P.F. (2019). A Novel In Vivo Infection Model To Study Papillomavirus-Mediated Disease of the Female Reproductive Tract. mBio, 10(2), e00180-19.

Publication 2019

RNAscope 2.5 HD Assay-Brown DNase I

Assay Cell Diagnostics Dnase i Hematoxylin Hybridization Protease Tissue

Corresponding Organization :

Other organizations : University of Wisconsin–Madison

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Protocol cited in 4 other protocols

Variable analysis

independent variables

DNase I treatment

dependent variables

Detection of MmuPV1 viral transcripts

control variables

Protease treatment
Probe hybridization
Hematoxylin counterstaining

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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