Cell Fractionation and Immunoblotting Protocol

Whole-cell lysates and separate nuclear/cytoplasmic fractions were extracted from cells according to routine protocols. Western blotting and coimmunoprecipitation were executed as preceding description [21 (link)]. The antibodies against GAPDH (60004-1-Ig), Flag-tag (20543-1-AP), α-tubulin (11224-1-AP), Lamin B1 (12987-1-AP), and GST-tag (HRP-66001) were purchased from ProteinTech. The antibodies against TNPO1 (ab10303, Abcam) and FUBP1 (ab181111, Abcam).

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Yang B., Chen J, & Teng Y. (2021). TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer. Journal of Immunology Research, 2021, 9994004.

Publication 2021

-tubulin (11224-1-AP), ab10303 ab181111

Antibodies Cell Coimmunoprecipitation Cytoplasmic Fubp1 Gapdh Lamin b1 Tnpo1 α tubulin

Corresponding Organization : Jiangsu University

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Variable analysis

independent variables

Whole-cell lysates and separate nuclear/cytoplasmic fractions

dependent variables

Protein expression levels measured by Western blotting
Protein-protein interactions measured by coimmunoprecipitation

control variables

Antibodies against GAPDH, Flag-tag, α-tubulin, Lamin B1, and GST-tag

positive controls

Not specified

negative controls

Not specified

Annotations

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